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Renal Infiltration of T Lymphocytes in a Rat Model of Polycystic Ovary Syndrome
Author(s) -
Moulana Mohadetheh,
Wallace Kedra,
Hosick Karen,
Lamarca Birdie,
Reckelhoff Jane
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb894
Subject(s) - medicine , endocrinology , polycystic ovary , placebo , cd8 , kidney , flow cytometry , dihydrotestosterone , hormone , ovary , immune system , immunology , insulin resistance , androgen , diabetes mellitus , pathology , alternative medicine
Polycystic ovary syndrome (PCOS) is the most common hormonal disorder among women of reproductive age. PCOS is strongly linked to increased risk of cardiovascular diseases (CVD). The mechanisms responsible for PCOS hypertension are not clear. We have characterized a rat model of PCOS, the hyperandrogenemic rat (HAF), that exhibits many of the characteristics of PCOS in women. This study aimed to test the hypothesis that PCOS rats exhibit increased T cells in kidney that could potentially play a role in hypertension. Dihydrotestosterone (DHT) (7.5 mg/90 days) or placebo, pellets were implanted subcutaneously in female SD rats (n = 4–5/group) at 4weeks of age and compared to placebo. After 6 weeks of DHT treatment, kidneys were collected and infiltrated CD4 + , CD8 + , Th17, and CD4 + CD28 null T cells were measured by flow cytometry. The number of renal infiltrated CD4 + T cells was increased in HAF rats (Placebo: 10 ±1 vs. HAF: 22 ±1 % gated p <0.05). The number of infiltrated Th17 (Placebo: 19 ±1 vs. HAF: 23 ±1 % gated p <0.05) and CD4 + CD28 null T cells (Placebo: 6 ±1 vs. HAF: 15 ±2 % gated p <0.05) were significantly increased in HAF rats compared to Placebo. These data show that infiltrated T cells, in particular Th17 and CD4 + CD28 null subpopulations are increased in the kidney of HAF rats, a model of PCOS. Results from this study suggest a potential role for infiltrated T cells as a link between hypertension and CVD in PCOS patients.