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Ablation of sympathetic neurons projecting to mesenteric blood vessels attenuates nicotine induced rise in blood pressure (BP)
Author(s) -
Hammond Bradley,
Kreulen David
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb840
Subject(s) - mesenteric arteries , ganglionectomy , venule , blood pressure , neurotoxin , population , sympathetic nervous system , anatomy , medicine , stimulation , endocrinology , biology , pathology , endothelium , artery , alternative medicine , environmental health
Celiac ganglionectomy attenuates the increase in BP seen in the DOCA‐salt model of hypertension; however, it is unknown which population of neurons in the celiac ganglion (CG) are responsible for this attenuation. Therefore, we ablated sympathetic post ganglionic neurons specifically innervating mesenteric blood vessels by applying a retrograde tracer, cholera toxin subunit b, conjugated to the neurotoxin saporin (CTB‐SAP) on two, adjacent branches of mesenteric arteries and veins. Control animals received PBS instead of a CTB‐SAP treatment. 1wk after CTB‐SAP application, rats were anesthetized, fitted with a femoral catheter, and the CG was dissected. Nicotine was then applied to the CG, which caused a significant increase in BP in control animals but not in CTB‐SAP treated animals. Immunohistochemistry of the CG revealed neuronal destruction in CTB‐SAP treated animals when compared to control animals. This study shows ablating CG neurons that innervate a small portion of the vasculature reduces the effect of CG stimulation on systemic BP, suggesting these neurons are likely to innervate a sufficient number of blood vessels to regulate systemic BP. Moreover, this study gives way for further investigations into the differing roles of separate neuronal sub populations in the CG. RC060365Blood Pressure (mmHg) Baseline Nicotine (200 mM)Control 74.6 ± 5.9 90.2 ± 6 * CTB‐SAP 99.3 ± 19.8 94.3 ± 9.2 ** p ≤ 0.05 to baseline