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Comparison of rectangular and sine waveforms on electrical stimulation to atrophy of rat soleus muscle
Author(s) -
Tanaka Minoru,
Tanaka Masayuki,
Kanazashi Miho,
Kondo Hiroyo,
Murakami Shinichiro,
Fujita Naoto,
Fujino Hidemi
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb829
Subject(s) - stimulation , hindlimb , atrophy , muscle atrophy , soleus muscle , anatomy , medicine , chemistry , endocrinology , skeletal muscle
PURPOSE We focused on the difference between rectangular and sine waves, investigated the effect of electrical stimulation on muscle atrophy. METHODS The rats were randomly divided into hindlimb unloading (HU), hindlimb unloading plus electrical stimulation with rectangular waves (HU+RS), hindlimb unloading plus electrical stimulation with sine waves (HU+SS), and weightmatched control (Cont) groups. In the HU+RS and HU+SS groups, the stimulation was percutaneously provided to calf muscles. After 14 days, the soleus muscle located in deep layer of calf was removed. RESULTS The value of muscle fiber cross‐sectional area was significantly less in the HU group than in the Cont group. Although there were no significant differences between the HU and HU+RS groups, the value in the HU+SS group was significantly larger than that in the HU and HU+RS groups. The expression level of ubiquitinated proteins was significantly higher in the HU group than in the Cont group, whereas the expression level was significantly less in the HU+SS group than in the HU and HU+RS groups. CONCLUSION The effect of electrical stimulation on muscle atrophy was depended on the waveform, and sine wave was effective in deep muscle. Supported by Grants‐in‐Aid for Science Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology.