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Protective effects of loading combined with astaxanthin on capillary regression of disused muscle
Author(s) -
Kanazashi Miho,
Tanaka Minoru,
Tanaka Masayuki,
Kondo Hiroyo,
Murakami Shinichiro,
Fujita Naoto,
Fujino Hidemi
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb818
Subject(s) - astaxanthin , hindlimb , muscle atrophy , soleus muscle , oxidative stress , atrophy , skeletal muscle , endocrinology , medicine , chemistry , biochemistry , carotenoid
PURPOSE Disuse‐induced muscle atrophy and capillary regression in skeletal muscle are related to overexpression of oxidative stress caused mitochondrial dysfunction. The purpose of the present study was to identify the effect of loading combined with astaxanthin on capillary regression of the soleus muscle induced by hindlimb unloading. METHODS Thirty‐five adult male rats were divided into control, hindlimb unloading (HU), HU+astaxanthin (Ax), HU+loading (Lo) and HU+Ax+Lo groups. RESULTS HU resulted in a decreased muscle mass and capillary regression. The loading attenuated the muscle atrophy, which increased the ROS and SOD‐1, resulting in a capillary regression. The combination of loading and astaxanthin attenuated the overexpression of oxidative stress. In addition, the combination increased the expression of e‐NOS, PGC‐1α and succinate dehydrogenase activity, reflecting a protective effect of mitochondrial function and the architecture of capillary network. CONCLUSION Loading combined with astaxanthin is effective in preventing capillary regression in the soleus muscle induced by hindlimb unloading. Supported by Grants‐in‐Aid for Science Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology.