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Effects of heat stress on mechanical ventilation‐induced atrophy in rat diaphragm
Author(s) -
IchinosekiSekine Noriko,
Yoshihara Toshinori,
Kakigi Ryo,
Sugiura Takao,
Powers Scott K,
Kawai Sachio,
Naito Hisashi
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb816
Subject(s) - tunel assay , diaphragm (acoustics) , atrophy , heat stress , chemistry , ventilation (architecture) , mechanical ventilation , medicine , apoptosis , zoology , biology , biochemistry , mechanical engineering , physics , engineering , acoustics , loudspeaker
This study investigated the effects of heat stress on mechanical ventilation‐induced atrophy in rat diaphragm. Adult male Wistar rats (n=38) were assigned randomly to control, heat stress, mechanical ventilation (MV), or heat stress + MV groups. Heat stress consisted of 1 h in a heat chamber set at 41°C, 24 h before MV. MV was performed for 12 h under anesthesia. Immediately after MV, diaphragm was removed, frozen and stored for analysis. All procedures were approved by the animal ethics committee of Juntendo University. MV significantly increased cleaved caspase‐3 (p<0.05) and reduced the mean muscle fiber cross‐sectional area (CSA, p<0.05). TUNEL index was not affected by MV. Heat stress resulted in a significant induction of heat shock protein72 (p<0.01), and attenuated reduction of CSA (p<0.05). Cleaved caspase‐3 and TUNEL index tended to be decreased by heat stress. These results indicate that 1 h whole‐body heat stress 24 h before MV may attenuate the acute diaphragmatic atrophy via an apoptotic pathway. This work was supported by a Grant‐in‐Aid for Young Scientists (B) No. 23700781 and JSPS Fellows No. 11J11122 by JSPS.