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ICAM‐1 expression by skeletal muscle cells augments stages of myogenesis
Author(s) -
Goh Qingnian,
Dearth Christopher L.,
Pizza Francis X.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb813
Subject(s) - myogenesis , skeletal muscle , microbiology and biotechnology , myocyte , c2c12 , biology , cytoplasm , intracellular , anatomy
We previously reported that intercellular adhesion molecule‐1 (ICAM‐1), an important protein of the inflammatory response, was expressed by satellite cells and myofibers of hypertrophying muscles. As skeletal muscle cell expression of ICAM‐1 contributed to the formation of regenerating fibers, as well as hypertrophy to mechanical overload, we speculate that ICAM‐1 expression by skeletal muscle cells facilitates myogenesis. Therefore, the purpose of the current study was to establish phenotypic alterations associated with skeletal muscle cell expression of ICAM‐1. Through stable transfection of C2C12 myoblasts with an ICAM‐1 plasmid, we found ICAM‐1 expression augmented stages of myogenesis in which myotubes are forming, adding nuclei, aligning, synthesizing proteins, and hypertrophying. As the cytoplasmic domain of ICAM‐1 is capable of activating several intracellular signaling pathways, we selectively inhibited the function of the cytoplasmic domain with a cell permeable peptide to reveal underlying mechanisms involved in ICAM‐1 mediated myogenesis. Here, we showed that the ICAM‐1 cytoplasmic domain contributed to enhanced rates of protein synthesis via increased Akt/p70s6k signaling. Hence, our findings extend knowledge of the immunobiology of skeletal muscle cells by revealing a novel mechanism through which the inflammatory response facilitates myogenesis.