Premium
GSK3Beta and LEF1 in regenerating young skeletal muscle following downhill running
Author(s) -
Arthur Susan Tsivitse,
Amin Hiral,
Vachris Judy,
Hamilton Alicia,
Howden Reuben,
Steuerwald Nury
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb803
Subject(s) - wnt signaling pathway , skeletal muscle , western blot , medicine , endocrinology , signal transduction , biology , microbiology and biotechnology , biochemistry , gene
The role of Wnt signaling during skeletal muscle repair has been studied using artificial models of muscle injury. Downhill running (DHR) is a physiological muscle injury model that involves a systems approach and is ideal when investigating sources of muscle repair. Purpose The objective of this study was to determine if DHR affects Wnt signaling. Methods C57BL/J6 male mice (3 mo) were exposed to either DHR or normal cage activity. The gastrocnemius was excised at 1, 2, 3, 4, 5, or 6 days post‐DHR. Gene (qRT‐PCR) and protein (western blot) expression of Wnt signaling components and GSK3□ (total and active) were measured. Results Muscle injury increased from baseline 0.4% to 1.4% at 3D, and 5D, and to 2.2% at 6D post‐DHR. M‐Cadherin protein increased from baseline (20.1%) to 54.7% at 3D through 5D (67.2%) post‐DHR. Total GSK3β protein decreased from baseline to 2D (3 fold), 3D (6 fold), 4D (3 fold) and 6D (3 fold) post‐DHR. GSK3βpY216 protein decreased from baseline to 2D (2.2 fold), 3D (3.7 fold), 4D (1.8 fold) and 6D (2.2 fold) post‐DHR. LEF1 protein increased from baseline to 5D and 6D (5 fold) post‐DHR. Conclusions In this study, DHR induced Wnt signaling activation. This physiological model provides insight into the complex role of Wnt signaling in skeletal muscle regeneration, which could be clinically important in treating skeletal muscle dysfunctions.