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Prolonged exercise upregulates expression of MCT1, MCT2, and MCT4 mRNA in the cerebral cortex of rat brain
Author(s) -
Takimoto Masaki,
Takeyama Mirei,
Hamada Taku
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb782
Subject(s) - messenger rna , endocrinology , medicine , transporter , chemistry , downregulation and upregulation , gene isoform , treadmill , cerebral cortex , monocarboxylate transporter , gene expression , biology , gene , biochemistry
Brain is capable of taking up monocarboxylates as energy substrate. The transport of monocarboxylic acids across cellular membranes is mediated by the H+/monocarboxylate transporters (MCTs). In the brain, three isoforms, MCT1, MCT2, and MCT4 have been identified. However, whether exercise upregulates the expression of MCT1, MCT2, and MCT4 at the mRNA level is unknown. We have examined the effect of a single exercise session on the mRNA expression of the MCT1, MCT2 and MCT4 in the cerebral cortex of rat brain. Cortex samples were obtained immediately and 5, 10, 18 and 24 h after 2 h of treadmill exercise (20 m/min, 8% grade). The expression of all MCTs mRNA did not change immediately after 2 h exercise bout. However, the expression of MCT1 and MCT2 mRNA significantly increased to control levels at 10 h after exercise, and, this increases maintained until 18 h after exercise. In contrast, MCT4 mRNA expression significantly increased at 18 h after exercise. These results suggest that a single bout of prolonged endurance exercise can transiently increase expression of MCT1, MCT2 and MCT4 mRNA in the brain as in the muscle of rat.