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Effect of melatonin combined with treadmill exercise on the cartilage regeneration of experimental osteoarthritic rats
Author(s) -
Jin Yunho,
Hong Yunkyung,
Hong Yonggeun
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb764
Subject(s) - ex vivo , osteoarthritis , chondrocyte , cartilage , endocrinology , medicine , population , chemistry , pathology , anatomy , in vitro , biochemistry , alternative medicine , environmental health
Osteoarthritis (OA) is a complex degenerative joint disease causing disability in the general population of world wide. The purpose of the study is to elucidate the effect of combined treatment of exercise (Ex) on melatonin (MT)‐induced cartilage regeneration in the experimental OA rats. OA in the 8‐week‐old Sprague‐Dawley rats was induced by intra‐articular collagenase injection. Then starting 1 day after OA induction, rats were injected subcutaneously with MT at doses of 10 mg/kg body weight, twice a day (07:00, 19:00), and Ex was conducted for 1‐hr/day. There was a significant reduction of not the joint width but the thickness in MT and/or Ex. Serum TNFa level was decreased by MT and/or Ex, whereas IL‐6 was increased by co‐intervention. Chondrocyte hypertrophic marker, Runx2, was attenuated in MT and/or Ex. LC3 II as well as MMP13 expression was down‐regulated by MT combined with Ex. Arrangement as well as morphology of chondrocyte improved in the cartilage of MT+Ex rats, which led to attenuation of stride width during gait (P<.05). Finally, it was shown in MT and Ex that no significance of the trabecular parameters between the damaged and undamaged joint. Therefore, we suggest that cartilage regeneration is induced by MT and Ex and that therapeutic effect is greater in the co‐intervention group than solely MT treated group.