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Cytoskeletal regulation dominates proteomic changes associated with hibernation in 13‐lined ground squirrels
Author(s) -
Hindle Allyson,
Epperson L Elaine,
Martin Sandra L
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb735
Subject(s) - torpor , hibernation (computing) , homeothermy , biology , proteome , zoology , ecology , biochemistry , thermoregulation , state (computer science) , algorithm , computer science
13‐lined ground squirrels are obligate hibernators that transition from summer homeothermy to winter heterothermy – wherein they exploit episodic torpor bouts. To explore the elements of winter neuroprotection, we applied 2D gel electrophoresis coupled with LC‐MS/MS to brain extracts of squirrels in two summer, four winter and fall transition states. Only 88 protein spots differed significantly among groups. The abundance pattern of the brain proteome was reciprocal between cold‐torpid and euthermic animals. The brain proteome of fall warm‐, but not cold‐torpid squirrels strongly resembled the homeotherms, indicating that the changes observed in torpid hibernators are defined by body temperature, not torpor per se. Metabolic enzymes were largely unchanged despite varied metabolic activity across annual and torpor‐arousal cycles. Proteins elevated in summer and aroused winter squirrels represented pathway enrichments in neural growth/differentiation and synaptic transmission. Differential cytoskeletal regulation across torpor‐arousal cycles is revealed by changes in microtubule dis/assembly regulating protein abundance (STMN1, DPYSL2), suggesting a mechanism for cytoskeletal stabilization during torpor and rapid reorganization on return to euthermy. Funded by NIH R01HL089049.