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Effect of glutamine supplementation and resistive training in signaling pathways of protein synthesis and degradation in rat skeletal muscle
Author(s) -
PithonCuri Tania C,
Rodrigues Carlos F,
Sousa Luis G O,
Vasconcelos Diogo A,
Vinolo Marco A,
Rodrigues Alice C,
Nachbar Renato T,
GuimarãesFerreira Lucas,
GerlingerRomero Frederico,
Sellitti Donald F,
Hirabara Sandro M,
Curi Rui
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb719
Subject(s) - glutamine , skeletal muscle , phosphorylation , protein kinase b , medicine , endocrinology , glutamine synthetase , protein degradation , blot , signal transduction , chemistry , biochemistry , amino acid , gene
The effect of physical training and glutamine supplementation on signaling pathways involved in protein synthesis (Akt, 4EBP ‐1 and S6) and degradation (FOXO, MuRF‐1 and MAFbx) of rat skeletal muscle was determined by real time PCR and western blotting in the following groups: 1) control (C); 2) trained (T); 3) control supplemented with glutamine (G); and 4) trained supplemented with glutamine (GT). Exercise protocol was conducted once every 3 days, during 5 weeks. Each training session consisted of six climbs in a vertical ladder (1.1 m; 80° from horizontal). EDL muscle mass was not changed, but a significant increase in the cross‐sectional area of the fibers was observed. Expression of FOXO‐1, Akt, atrogin‐1, Murf‐1, 4E‐BP1, and S6 was not altered. In contrast, phosphorylated S6 and 4EBP‐1 expression was significantly increased in the G and GT groups, and 26S proteasome activity decreased in the T group as compared to C group. Our results suggest that glutamine supplementation led to an increase of the phosphorylation of state 4EBP‐1 and S6. The 5 weeks of a physical training protocol described in this study in rats is a useful model to investigated the molecular mechanisms underlying the increased muscle mass due to long‐duration exercise. This research was supported by FAPESP, CNPq, CAPES and Guggenheim Foundation.