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Study of the roles of LPA3 on erythropoiesis and thrombocytopoiesis processes in zebrafish
Author(s) -
LI MENG WEI,
Huang ChangJen,
Lee Hsinyu
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb714
Subject(s) - erythropoiesis , zebrafish , haematopoiesis , lysophosphatidic acid , chemistry , receptor , microbiology and biotechnology , platelet , medicine , endocrinology , biology , stem cell , immunology , biochemistry , anemia , gene
Study of the roles of LPA3 on erythropoiesis and thrombocytopoiesis processes in zebrafish Hematopoiesis is a dynamic process regulated by complicated pathways. It is responsible for the production of hematopoietic stem cells, as well as their differentiation into more specific mature cell types. Lysophosphatidic acid (LPA), an extracellular lipid mediator, binds to its cognate G protein‐coupled receptors, through which can regulates differentiation and development of the circulation system. In previous study, we demonstrated that LPA regulates hematopoiesis determination in stem cells and erythropoiesis processes through activating LPA receptor 3 (LPA3). Since erythrocytes and thrombocytes were derived from common progenitors, we aim to investigate whether LPA3 also regulates thrombocyte development. Using zebrafish as a model, we found that morpholino (MO)‐mediated knockdown of LPA3 inhibited production of hemoglobin using o'dianisidine staining of zebrafish embryos. Furthermore, treatment with OMPT, a LPA3 agonist, and Ki16425, a LPA1 and LPA3 antagonists, were used to determine if the differentiation process of erythrocyte or thrombocyte is affected. Our results show that treatment with Ki16425 leads to erythropoiesis defects. In addition the expression levels of CD41 (αIIb, glycoprotein IIb [GPIIb]), a marker for thrombocyte, was increased by LPA3 MO and decreased by with OMPT treatments. In summary, our results demonstrated that LPA3 is an important regulator for erythropoiesis and thrombocytopoiesis process of hematopoietic stem cells.