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Skeletal muscle protein degradation induced by high‐fat diet is decreased by macadamia oil supplementation: Role of E3 ubiquitin enzyme ligase MuRF‐1 and atrogin‐1
Author(s) -
Hirabara Sandro M,
Sousa Luis G O,
Rodrigues Carlos F,
Martins Amanda R,
Neto Jose C Rosa,
Rodrigues Alice C,
Lima Edson A,
Gorjao Renata,
PithonCuri Tania C,
Curi Rui
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb706
Subject(s) - endocrinology , medicine , skeletal muscle , muscle atrophy , ubiquitin ligase , ubiquitin , protein degradation , chemistry , enzyme , biology , biochemistry , gene
This study aimed to assess the effect of macadamia oil (MO) on expression of genes involved in proteolytic pathways (Murf‐1 and Atrogin‐1) in diet‐induced obese C57/BL6J mice. Mice were fed a control or a high‐fat diet (HFD) and supplemented or not with MO (2.5 mg/kg) for eight weeks. mRNA levels of MuRF‐1 and atrogin‐1/MAFbx were evaluated in EDL muscle by real‐time PCR. Mice fed a HDF presented decreased EDL weight when compared to control diet (0.28 vs 0.39, respectively), suggesting muscle atrophy. MO supplementation decreased this effect (0.28 vs control 0.34; p<0.05). A significant two‐fold increase in mRNA levels of MuRF‐1 and atrogin‐1/ MAFbx, in EDL muscle, was found in mice fed a HFD. On the other hand, MO supplementation restored mRNA levels of Murf‐1 and Atrogin‐1 in mice fed a HFD to control levels, suggesting a protective effect on the pathways involved in the HFD‐induced proteolysis. Our data suggest that the increased skeletal muscle degradation induced by HDF is decreased by MO supplementation by a mechanism that involves reduced RNAm expression of Murf‐1 and Atrogin‐1. Support: FAPESP, CNPq, CAPES, Dean's Office for Research/USP, Clear, INOD, and NEL.