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Identification of a quinone compound inhibits human glutaminase activity and induces autophagy in carcinoma cells
Author(s) -
Lee ShiowJu,
Lee YueZhi,
Hsu HsingYu,
Chang HsinYu,
Chen IhSheng,
Chao YuSheng
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb70
Subject(s) - autophagy , natural product , glutaminase , quinone , a549 cell , biology , cancer research , christian ministry , apoptosis , chemistry , microbiology and biotechnology , biochemistry , glutamine , philosophy , theology , amino acid
Natural product sources recently have been applied to discover drugs for being developed to treat cancer, immunosuppressive disorders, resistant bacteria and viruses. In many instances, the disease targeted natural products serve as tools to unravel the cellular molecular targets and pathways in the disease processes and thus facilitate further development. Here, we identified a natural quinone compound that inhibited human kidney glutaminase activity and the cell growth and proliferation of lung carcinoma A549 and hepatoma HepG2 as well as blocked the anchorage‐independent colony formation of HepG2. Autophagy but not apoptosis was found in A549 and HepG2 cells treated with the natural quinone compound. Further investigation demonstrated the natural quinone compound inhibiting glutaminase activity and conceivably depleting the cellular nutrient and energy to induce autophagy for anti‐cancer activity. (This work was funded by the National Health Research Institutes, Taiwan, R.O.C. and by the Ministry of Economic Affairs, R.O.C. “101‐EC‐17‐A‐02–04‐1099” and “102‐EC‐17‐A‐02–04‐1099”)