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Effects of Reduced Physical Activity on Intracellular Nitric Oxide in Circulating Angiogenic Cells
Author(s) -
Guhanarayan Gayatri,
Jablonski Julianne,
Witkowski Sarah
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb659
Subject(s) - hemocytometer , cd34 , nitric oxide , chemistry , endothelium , population , peripheral blood mononuclear cell , intracellular , medicine , endocrinology , immunology , andrology , microbiology and biotechnology , biology , biochemistry , stem cell , in vitro , environmental health
Physical inactivity, a risk factor for cardiovascular disease, promotes changes in the endothelium that may be influenced by circulating angiogenic cells (CACs). CACs have the capacity to replace and repair damaged vascular endothelium. Intracellular nitric oxide (NO i ) is essential for CAC migration, motility, and proliferation and is therefore a key determinant of CAC health. The objective was to determine whether CAC number and NOi levels changed in response to ten‐days of reduced physical activity (rPA). Lipid profile, weight, and blood pressure (BP) were quantified before and after rPA. rPA was confirmed with accelerometry and caloric intake was reduced to match the reduced energy expenditure during rPA. CACs (CFU‐Hill, CD34 + ) were isolated from PBMCs prior to and following rPA. CD34 + cells were isolated via magnetic separation and quantified with a hemocytometer. CFU‐Hill were cultured and quantified via colony‐forming assay. NO i was determined using DAF‐FM diacetate. There were no significant changes in lipid profile, weight, or BP with rPA. CFU‐Hill colonies decreased by 44.7% and CD34 + cells increased by 34.8% (p=0.095, p=0.45, respectively). CFU‐Hill NO i was significantly decreased by 52.7% (p=0.023) and CD34 + cells had reduced NO i by 24% (p=0.37). The effect of rPA on CAC NO i is specific to the CAC population. UMass, Amherst Faculty Research Grant and CHC Research Grant