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Aging augments apoptosis of endothelial cells in mice
Author(s) -
Campagnaro Bianca Prandi,
Tonini Clarissa Loureiro,
Porto Marcella Leite,
Vasquez Elisardo Corral,
Meyrelles Silvana Santos
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb648
Subject(s) - apoptosis , propidium iodide , annexin , apolipoprotein e , flow cytometry , pi , analysis of variance , andrology , chemistry , endocrinology , medicine , biology , immunology , programmed cell death , biochemistry , disease
We evaluate mice endothelial cells (EC) apoptosis in aging and atherosclerosis by flow cytometry. Male apolipoprotein E‐deficient (ApoE, N=10) mice were separated in two groups according to their ages: 2 and 18 months old and compared with C57BL/6 mice (C57, N=10). Animals were euthanized and EC mechanically isolated from aorta. For apoptosis analysis, 105 cells were incubated with Annexin V‐FITC and propidium iodide (PI) at room temperature during 15min. Events were acquired by FACSCanto II and analyzed by FACSDiva software. Data are means±SEM. p<0.05 (two‐way ANOVA). Flow cytometric analysis showed that atherosclerosis did not affected EC apoptosis between young (C57: 1.9±0.2 vs. ApoE: 1.15±0.12 %) and aged (C57: 23.25±7.7 vs. ApoE: 23.36±0.55 %) groups. However, aging augments apoptosis of EC in ApoE (Young: 1.15±0.12 vs. Aged: 23.36±0.55 %) and C57 (Young: 1.9±0.17 vs. Aged: 23.25±7.71 %) groups. Our data showed that atherosclerosis per se was not able to induce apoptosis. However, aging was directly responsible for augmentation of EC apoptosis.