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Adhesive Interaction of Functionalized Particles and Endothelium in Idealized Microvascular Networks
Author(s) -
Lamberti Giuseppina,
Tang Yuan,
Prabhakarpandian B.,
Wang Yi,
Pant Kapil,
Kiani Mohammad F.,
Wang Bin
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb641
Subject(s) - adhesion , adhesive , endothelium , chemistry , particle (ecology) , biophysics , materials science , composite material , layer (electronics) , biology , ecology , endocrinology
Objective Leukocytes response to tissue injury/infection is mediated by a series of interactions between receptors and ligands on the endothelium and the leukocytes. Here, we characterize the adhesion profile of functionalized particles under physiological flow conditions in an idealized synthetic microvascular network (SMN) characterized by a bifurcation. Methods Functionalized particles were prepared by coating their surfaces with different ratios of antibodies against ICAM‐1 and E‐selectin (aICAM‐1:aE‐selectin = 100:0, 70:30, 50:50, 30:70, 0:100). The adhesion of functionalized particles to 4hr TNF‐α activated HUVECs under shear flow (0.5, 2, and 4 dyn/cm 2 ) in bifurcating SMNs was then quantified. Results The level of adhesion of 50:50 aICAM‐1:aE‐selectin particles was significantly higher compared to other particles in the bifurcating SMNs (~1.5–4 folds higher). Furthermore, the adhesion of particles in junction regions was about 3–18 folds higher than that in straight sections of the SMNs. Particle adhesion did not change significantly with decreasing shear at the junction regions of SMNs for all functionalized particles. Conclusion The differential adhesion patterns of particles between straight sections and junction regions of bifurcating SMNs, indicates that adhesion profile of particles is highly dependent on the vascular geometry of the system used.