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Effects of alpha, beta‐unsaturated cinnamyl‐like aromatic compounds on the viability of a model human cancer (MCF‐7) cell line
Author(s) -
Muhammad Yosra,
Kerr Stephen G
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb637
Subject(s) - chemistry , glutathione , cytotoxicity , viability assay , cytotoxic t cell , cinnamaldehyde , cell culture , biochemistry , cell , enzyme , in vitro , biology , genetics , catalysis
Cinnamyl‐like aromatic compounds, like trans ‐cinnamaldehyde (CA) and methyl cinnamyl ketone (MeCK), are α,β‐unsaturated carbonyl compounds that are believed to interact with cellular nucleophiles through a Michael addition. The Michael acceptor structure of CA and MeCK makes them potential therapeutic alkylating agents. This study investigated the effects of CA and MeCK on the viability of MCF‐7 cells, alone and in the presence of ethacrynic acid (EA), a diuretic and glutathione‐S‐transferase (GST) inhibitor, as well as glutathione (GSH). MCF‐7 cells were seeded in a 96‐well plate (10 4 cells/well/100μl), and treated with CA (10—250 uM) and MeCK (100—500 uM) alone, and in combination with EA (50 uM) or GSH (100 uM) for 24 h at 37 ºC in 5% CO 2 . MTS cell viability assays were performed to determine the percentage viability of the treated cells relative to that of the control cells. The results indicate that CA at low concentrations (10–50 uM) induced cell growth, while at concentrations of ≥ 100μM, it was cytotoxic to MCF‐7 cells in a dose‐dependent manner. In the co‐treatment studies, GSH provided protection to cells against MeCK activity, but not against CA. The presence of EA greatly enhanced the cytotoxicity of both CA and MeCK. These results suggest that the cinnamyl‐like aromatic compounds, CA and MeCK, have the potential to induce cell proliferation at low concentrations and to cause cytotoxicity at high concentrations. In addition, the cytotoxic effect was attenuated by co‐treatment of GSH with only MeCK, but enhanced in the presence of EA.