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DETERMINATION OF BPA AND ITS METABOLITES BY HPLC‐UV‐Vis AND MALDI‐TOF
Author(s) -
Naidu Naga,
Munyu Sarah,
Yakubu Momoh A.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb636
Subject(s) - urine , chemistry , chromatography , high performance liquid chromatography , feces , bisphenol a , metabolite , biology , biochemistry , paleontology , organic chemistry , epoxy
Bisphenol A (BPA) is a weak estrogen and is of great environmental concern We have determined free BPA and its metabolites in urine, serum and feces samples using HPLC and MALDI‐TOF. Samples were collected from rats treated with 160 mg/kg BPA orally; BPA and its metabolites were extracted from urine, serum, and feces into acetonitrile and subjected to HPLC analysis. HPLC spectrum of standard BPA peak (RT 7.7) was determined and compared with the samples’ (R.T 7.5–7.7). HPLC spectra of samples confirmed the presence of BPA and its metabolites in different concentrations in urine (8.22 – 24.93ppm), serum (1225.30 – 1868.61ppm) and feces samples (3.48 – 29.95 ppm). No peaks corresponding to BPA was detected in controls. To confirm BPA and metabolites in the samples, eluent from R.T 7–9 was collected and analyzed on MALDI‐TOF. MALDI‐TOF analysis of standard BPA showed a peak at 223.8 m/z, urine samples showed three minor peaks at 223.8 m/z and 245 m/z and 415 m/z corresponding to free BPA, quinine and glucoronide conjugates; a major peak at 205.78 m/z representing BPF, a byproduct of BPA. Serum samples showed minor peaks at 223.68 m/z and 490.54 m/z corresponding to free BPA and BPA‐glu conjugate. Feces samples showed major peak at 215 m/z. Thus, HPLC‐UV‐Vis is a very sensitive tool for identifying BPA and its metabolites in urine and feces samples and confirmed by MALDI‐TOF.