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Effects of glucose concentration on propofol cardioprotection against ischemia‐reperfusion injury in isolated rat hearts
Author(s) -
Tao Mingzhe,
Liu Huimin,
Zhang Ping,
Xia Zhengyuan
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb620
Subject(s) - cardioprotection , propofol , oxidative stress , medicine , ischemia , pharmacology , cardiac function curve , reperfusion injury , anesthetic , cd36 , anesthesia , chemistry , heart failure , receptor
The anesthetic propofol protects against myocardial ischemia‐reperfusion injury (IRI) by reducing reactive oxygen species (ROS)‐induced oxidative stress. However, its cardioprotection in patients is inconsistent. We postulated that low glucose(LG) as seen during tight glycemic control promotes cardiac ROS formation through enhancing fatty acid(FA) oxidation during IRI and unmasks propofol cardioprotection. Rat hearts were isolated and randomly assigned to be perfused with Krebs‐Henseleit solution with glucose at 5.5 mmol/l (LG) in the absence or presence of propofol (5ug/ml) or propofol with trimetazidine (TMZ) unknown to inhibit FA oxidation, or with glucose at 8 mmol/L (group G) in the absence or presence of propofol or propofol plus TMZ. Hearts were subjected to 35 min of global ischemia and 60 min of reperfusion. Myocardial infarct size(IS) was higher in LG than in G group (P<0.05), accompanied with reduced left ventricular (LV) developed pressure and increases in postischemic cardiac contracture. Cardiac 15‐F2t‐isoprostane was higher in LG that was associated with higher cardiac lipid transporter CD36 protein expression than in G group. Propofol reduced IS and improved cardiac function and reduced cardiac CD36 in G but not in LG group. TMZ facilitated propofol cardioprotection in LG. During IRI, LG through enhancing fatty acid oxidation and oxidative stress compromised propofol cardioprotection.