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2K‐1C Hypertensive Rats Treated With Tempol Show Decreased MMP‐2 Activity Possibly Due To Oxidative Stress Blockage
Author(s) -
Antonio Raquel Carros,
Ceron Carla Speroni,
Rizzi Elen,
Santos José Eduardo Tanus,
Gerlach Raquel Fernanda
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb609
Subject(s) - nitrotyrosine , oxidative stress , reactive oxygen species , matrix metalloproteinase , zymography , antioxidant , chemistry , gelatinase , nitrosylation , medicine , endocrinology , pharmacology , biochemistry , nitric oxide , enzyme , nitric oxide synthase
Hypertensive structural and functional modifications are associated with increased MMP‐2 activity and reactive oxygen species (ROS). ROS can induce post‐translational modifications in proteins, such as nitrosylation, that are known to affect anzyme activity. ROS can also directly activate MMPs. This study examined whether the treatment with Tempol prevents raised systolic blood pressure (SBP) and the following biochemical findings of hypertensive heart tissue: ROS activity, total gelatinolytic activity, MMP‐2 activity, and presence of nitrotyrosine residues. Study protocol: 2kidney‐1clip (2K‐1C) rats and Sham‐operated rats (Sham) were treated with vehicle or Tempol (T) (18 mg/kg/day). Tested used were in situ oxidative stress; in situ zymography; immunofluorescence for MMP‐2 and nitrotyrosine. Tempol attenuated hypertension (2K‐1C: 193± 3mmHg; 2K‐1C+T; 159± 2mmHg; p<0,05), and led to decreases in ROS, total gelatinase activity, MMP‐2 activity and amount of stained nitrotyrosine in cardiac tissue (p<0,05 for all comparisons between the 2K‐1C group versus 2K‐1C+T). Thus, nitrosylation of MMP‐2 or of its substrates may change MMP‐2 activity in hypertension. Results show one important benefit of the treatment with the antioxidant Tempol that needs better understanding. This study was supported by Conselho Nacional de Pesquisa (CNPq) Brasil.