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Vasorelaxant activity of p‐cymene in superior mesenteric artery of rats
Author(s) -
Barbosa Renata Lisboa,
Santos Julyane Hora,
Cunha Patricia Santos,
QuintansJúnior Lucindo José,
Bonjardim Leonardo Rigoldi,
Souza Araújo Adriano Antunes
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb599
Subject(s) - phenylephrine , chemistry , mesenteric arteries , tetraethylammonium , endothelium , incubation , medicine , endocrinology , pharmacology , artery , potassium , biochemistry , organic chemistry , blood pressure
p‐Cymene is an alkylbenzene related to a monoterpene very promising for their biological properties, including actions on the cardiovascular system. Given this, the aim of the present study was to evaluate the vasorelaxant effect induced by p‐cymene in rat mesenteric artery. Artery rings with functional endothelium pre‐contracted with phenylephrine (PHE) (control), p‐cymene (10–8 – 3 × 10–2M) induced relaxation (Emax = 104 ± 6 %; n= 6) was not different from that obtained after removal of endothelium (Emax = 95 ± 7 %; n= 6) or pre‐contracted with KCI 80 mM (Emax = 72 ± 4 %; n= 6), suggesting a possible involvement of Ca2 + channels. In preparations without functional endothelium and pre‐contracted with PHE, incubation with tetraethylammonium, a non‐selective blocker of potassium channels, was able to reduce relaxations induced by p‐cymene in concentrations 10–3, 3.10–3, and 10–2M (p < 0.05 and p < 0.01). Furthermore, the incubation with p‐cymene (3 × 10–3, 10–2, and 3 × 10–2 M) was able to antagonize the contractions induced by CaCl2 (from 10–7 to 3 × 10–2 M) and sodium orthovanadate (from 10–5 to 3 × 10–2 M), an general inhibitor of protein‐tyrosine phosphatase. Taken together, these results suggest that p‐cymene produces a vasorelaxant effect by an endothelium‐independent mechanism in the rat mesenteric artery, possibly involving voltage sensitive Ca2+, K+ channels and Ca2+ desensitization of contractile elements. Support: CNPq, Capes, Fapitec