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Anti‐cancer activity of protocatechualdehyde through HDAC2‐mediated downregulation of cyclin D1 in human colorectal cancer cells
Author(s) -
Jeong Jin Boo,
Lee SeongHo
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb578
Subject(s) - cyclin d1 , cancer research , cell growth , downregulation and upregulation , apoptosis , cancer , chemistry , cyclin d , cancer cell , colorectal cancer , biology , cell cycle , biochemistry , genetics , gene
There are a few studies that protocatechualdehyde (PCA), a naturally occurring polyphenol in barley, induces cell growth arrest in breast and leukemia cancer cells. However, the underlying mechanisms are still poorly understood. Thus, we performed in vitro study to investigate whether PCA affects cell proliferation and apoptosis in human colorectal cancer cells and define potential mechanisms by which PCA induces growth arrest and apoptosis in human colorectal cancer cell lines, HCT116 and SW480. Exposure of PCA to HCT116 and SW480 cells suppressed cell growth and induced apoptosis in a dose‐dependent manner. PCA decreased cyclin D1 expression in protein and mRNA level and suppressed luciferase activity of cyclin D1 promoter, indicating a transcriptional downregulation of cyclin D1 gene by PCA. We also observed that PCA decreased enzyme activity of HDAC and reduced expression of HDAC2, but not HDAC1. These findings suggest that PCA results in cell growth inhibition and apoptosis via HDAC2‐mediated cyclin D1 suppression. This work was supported by start‐up funds (University of Maryland) to S‐H Lee.