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Patchouli Alcohol Possesses Anti‐Inflammatory Activity in RAW264.7 and HT‐29 cells
Author(s) -
Jeong Jin Boo,
Shin Yong Kyu,
Lee SeongHo
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb561
Subject(s) - patchouli , pogostemon , nf κb , tumor necrosis factor alpha , mapk/erk pathway , chemistry , downregulation and upregulation , macrophage , pharmacology , kinase , in vitro , biochemistry , endocrinology , apoptosis , biology , medicine , traditional medicine , essential oil , chromatography , gene
Patchouli alcohol (PA) is a chemical compound found in essential oil of patchouli which belongs to the genus Pogostemon, herb of mint family. Although it has been reported that PA suppresses the production of inflammatory mediators, the biological mechanisms of PA for anti‐inflammatory activities have not been studied. Thus, in this study, we investigated whether PA decreases the production of inflammatory mediators through downregulation of the NF‐¥êB and ERK pathway. Our data indicated that PA inhibits the expression of iNOS and IL‐6 in protein and mRNA levels in LPS‐stimulated RAW264.7 and TNF‐¥á stimulated HT‐29 cells. PA reduced I¥êB‐¥á degradation and p65 nuclear translocation, and subsequently suppressed transcriptional activity of NF‐¥êB in LPS‐stimulated RAW264.7 and TNF‐¥á‐stimulated HT‐29 cells. In addition, PA ameliorated LPS‐ or TNF‐¥á‐stimulated ERK1/2 activation. These findings suggest that PA possesses anti‐inflammatory activities through suppressing ERK‐mediated NF‐¥êB pathway in mouse macrophage and human colorectal cancer cells. This work was supported by start‐up funds to S.‐H. Lee from University of Maryland and in part by RSG‐11–133‐01‐CCE to S.‐H. Lee from American Cancer Society.