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New tools of regenerative medicine to promote wound healing
Author(s) -
Ippoliti Flora,
Canitano Nicoletta,
Azzara Gabriella,
Cannizzaro Cristina,
Corsi Mariangela,
Capoano Raffaele,
Salvati Bruno,
Businaro Rita
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb55
Subject(s) - medicine , wound healing , hyaluronic acid , platelet rich plasma , regeneration (biology) , platelet , surgery , diabetic ulcers , diabetes mellitus , pathology , diabetic foot , anatomy , biology , microbiology and biotechnology , endocrinology
STUDY OBJECTIVE The rationale of our study was to test the potential of the autologous elements of peripheral white blood cells and platelets to promote neoangiogenesis, thereby overcoming hypoxemia, the major obstacle to the regeneration of the tissue. MATERIALS AND METHODS An highly innovative technique, based on an autologous leuco‐platelet preparation, applied by direct infiltration to the ulcer was employed to treat 65 patients with ulcers of different etiologies. Hyaluronic acid was used as a scaffold, supplemented with autologous cells. Patients were followed up until complete healing of the lesions. Morphometric analysis was perfomed on histologic sections to determine the presence of neoangiogenesis. RESULTS The wound healing was obtained in all cases after 2 until 22 months with this treatment (median time is 11.4 months). More than half of the patients recovered completely within the first 12 months. Morphometric analysis on histologic sections determined the presence of an abundant neovasculature in the close proximity of the infiltrations of leuco‐platelet concentrate. CONCLUSIONS Our data show that autologous leucoplatelet preparation layered onto ischemic ulcers may improve neoangiogenesis leading to wound healing. These findings suggest that this cell‐based therapy may be a useful tool for the treatment for intractable skin ulcers resulting from diabetes, ischemia and collagen diseases