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Immunolocalization of Interleukin‐17 and Interleukin‐23 within human aortic aneurysms
Author(s) -
Ippoliti Flora,
Ricci Serafino,
Azzara Gabriella,
Di Gioia Cira,
Di Cristofano Claudio,
Massoni Francesco,
Onofri Emanuela,
Cassese Mauro,
Businaro Rita
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb486
Subject(s) - adventitia , elastin , medicine , aortic aneurysm , pathology , h&e stain , aorta , van gieson's stain , inflammation , interleukin , aneurysm , thoracic aortic aneurysm , thoracic aorta , proinflammatory cytokine , immunohistochemistry , cytokine , immunology , surgery
STUDY OBJECTIVE Much recent experimental evidence suggests that inflammatory reactions are involved in aneurysm formation and progression. Vessel wall weakening has been attributed to the infiltration of a variety of cells, which leads to the upregulation of multiple cytokines. A growing interest is currently focused in the definition of cytokines associated with the final stage of the disease that precedes the rupture. MATERIALS AND METHODS In the present study full aortic segments were collected from patients with thoracic aortic aneurysms, according to the declaration of Helsinki. Control aorta tissue from organ donors was included as reference. Microtome sections of paraffin embedded samples were analyzed for the presence of IL‐17 and IL‐23 positivity. Aortic sections were also stained with hematoxylin and eosin and Verhoeff‐Van Gieson for elastin. RESULTS Immunohistochemistry detected IL‐17 and IL‐23 positive cells at the level of adventitia and muscle cell layer. CONCLUSION Our data reinforce previous results showing that IL‐17 plays a central role in the promotion of vascular inflammation. The identification of main cytokines released during inflammatory processes underlying aneurysm formation and progression may lead to the set up of pharmaceutic strategies that may prove effective for the stabilization of aortic aneurysms.