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Complement MAC expression in heart failure
Author(s) -
Rietdorf Emily,
Chow Sheryl,
O'Barr Stephen
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb480
Subject(s) - heart failure , complement (music) , myocardial infarction , medicine , blot , cardiology , antibody , complement system , occlusion , immunology , biology , gene , biochemistry , complementation , phenotype
Complement is expressed during acute myocardial infarction (AMI), but little is known of its involvement in the development of heart failure (HF). Methods Rats were induced with HF by occlusion of the LAD coronary artery, and then sacrificed four weeks later. Controls were given a sham operation with the same procedure minus the occlusion. Hearts were excised then homogenized in RIPA buffer. Western blots were run with C7 (Quidel) and C5b‐9 (Hycult) antibodies at 1:1000, secondary antibody at 1:5000, blocked with TBST + 5% milk. Results Eight control samples vs. eight HF samples were evaluated. Both C7 and C5b‐9 levels in the HF samples were significantly increased in comparison with the controls. Conclusions These results demonstrate that complement is activated in our HF model, but more studies are needed to determine its specific role in HF pathology. Financial support was provided by the Drew Collaborative Grant # 12304P (SLC) , GCRC LaBioMed #00543–38F (SLC), NIH R21DK070003–01A1, and Western University of Health Sciences MSPS Graduate Program (ER).