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Asymptomatic African Americans with high‐risk APOL1 genotypes have reduced urinary angiogenesis‐promoting cytokines
Author(s) -
Gutierrez Jean Lorraine,
Many Gina,
Panchapakesan Karuna,
Brown Kristy J,
Hathout Yetrib,
Marathi Ramya,
Devaney Joseph,
Hoffman Eric P
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb474
Subject(s) - asymptomatic , medicine , urinary system , urine , creatinine , kidney disease , genotype , cytokine , risk factor , kidney , renal function , immunology , endocrinology , biology , genetics , gene
Background African Americans (AA) having two high‐risk APOL1 alleles, termed G1 and G2, are about 7 times more likely to develop non‐diabetic kidney diseases, and ApoL1 is differentially expressed in glomerular arterial compartments in those with overt kidney disease. Objective To compare angiogenic cytokines in the urine of young, asymptomatic AA with high‐risk for CKD (G1 homozygotes) to lower‐risk AA (non‐carriers and heterozygotes). Methods Thirty‐seven previously genotyped participants (5 G1 homozygotes, 20 WT homozygotes, and 12 heterozygotes) donated mid‐stream, first morning urine samples. Cytokine concentrations in unprocessed urine samples were analyzed with Milliplex Human Cytokine/Chemokine Panel bead assay. Urine cytokine concentrations were standardized to urinary creatinine, and the two risk groups were compared using the Mann‐Whitney U Test (SPSS Version 21). Results Concentrations of the following angiogenic cytokines were significantly lower in high‐risk G1 homozygotes: TGF‐α (P=0.004), PDGF‐AA (P=0.016), IL‐1‐alpha (P=0.032), VEGF (P=0.036). Conclusions This pilot data indicates that young, asymptomatic African Americans with high‐risk APOL1 genotypes may have impaired angiogenesis. These results are consistent with prior evidence indicating that vascular disease may underlie ApoL1‐associated kidney diseases. This study was funded by a CTSA pilot award.