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Osteopontin immunodetection after lateral luxation of developing molars in young alendronate‐treated rats
Author(s) -
AranaChavez Victor E,
Rothbarth Claudia Pires,
BradaschiaCorrea Vivian,
Ferreira Lorraine Braga,
Rezende Eloiza,
SilvaeSouza Patricia Almeida
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb466
Subject(s) - molar , cementum , dentin , osteopontin , dentistry , maxilla , chemistry , immunolabeling , saline , anatomy , medicine , immunohistochemistry , pathology
The aim of this study was to investigate the effects of alendronate (ALN) on developing molars after lateral luxation. 21 day‐old rats had their second upper molars laterally luxated. Daily 2.5 mg/kg ALN injections started at the day of luxation; controls received sterile saline. After 7, 14 and 21 days of luxation, the maxillae were fixed, decalcified and embedded in paraffin. The sections were stained with H&E, incubated for TRAP histochemistry or immunolabeled for osteopontin (OPN). After 21 days, the root apex of the luxated molars without ALN was wide open, disorganized, and covered by an irregular layer of cellular cementum. The luxated molars from ALN‐treated rats presented some ankylosis sites, as well as some resorptive lacunae at their root surface. The TRAP positive osteoclasts were more numerous in the ALN group that in controls, despite their latent appearance. OPN immunolabeling revealed a thick immunopositive line in the dentin that must be resultant from the moment of the luxation; contrarily, the ALN treated specimens did not present alterations in dentin. The findings indicate that ALN prevents that alterations in dentin and in cementum formation may be induced by dental trauma. Supported by CAPES‐DINTER (Brazil).