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Expression of miR‐125b and miR‐145 in breast cancer with overexpression of MUC1
Author(s) -
Zanetti Juliana Silva,
Darin Patricia R,
De Lucca Fernando,
RibeiroSilva Alfredo
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb459
Subject(s) - muc1 , microrna , immunohistochemistry , breast cancer , cancer research , cancer , biology , pathology , oncology , medicine , gene , genetics
The deregulation of microRNAs (miRNAs) expression is associated with a variety of human diseases including cancer. Recently, it was found that the mucin1 (MUC1) oncoprotein is a direct target of miR‐125b and miR‐145 in breast cancer cells established from invasive ductal carcinoma (IDC). It is known that data obtained using cancer cells are not always validated in intact tumor tissues. The aim of this study was to investigate the expression of miR‐125b and miR‐145 in IDC formalin fixed paraffin embedded (FFPE) tissues with overexpression of MUC1. The FFPE samples of IDC were selected by immunohistochemistry and the expression of miRNAs was evaluated by real time PCR. The normal breast FFPE samples were used as control. Our results indicate that miR‐125b and miR‐145 expression was reduced 40% (P<0.05) and 60% (P<0.05), respectively. These findings suggest that miR‐125b and miR‐145 negatively regulate the expression of MUC1 in tumor tissue from patients with IDC. Thus, this study may contribute to a better understanding of the molecular mechanisms involved in the regulation of MUC1 expression in breast cancer and to develop new therapeutic strategies for IDC.