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Investigation of immunological changes in mice subjected to animal model of sepsis
Author(s) -
Jeremias Isabela Casagrande,
Victorino Vanessa Jacob,
Barbeiro Hermes Vieira,
Lima Thais Martins,
Soriano Francisco Garcia
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb451
Subject(s) - sepsis , immune system , spleen , flow cytometry , cytotoxic t cell , medicine , immunology , population , encephalopathy , animal model , t cell , biology , in vitro , biochemistry , environmental health
Sepsis induces multiple organ dysfunction syndrome including septic encephalopathy, that is commonly seen in systemically ill patients. Moreover, knowing that changes in the nervous system are implicated in the induction or amplification of immune system changes, or conversely, and they are not well elucidated in sepsis, we investigate the changes in brain function and immune system of mice in an animal model of sepsis by cecal ligation and puncture (CLP). Balb/c mice underwent CLP and were divided into two groups: CLP or Sham operation. We evaluated brain changes by SHIRPA behavioral test, after 6h of CLP. Mice were sacrificed, spleen were isolated and evaluated by flow cytometry profile of population of lymphocytes. We observe that the neuropsychiatric state in SHIRPA test was significantly decreased in animals with CLP. Regarding lymphocytes, no difference in the number of cells (B lymphocytes, regulatory T cells, helper T lymphocytes and cytotoxic T lymphocytes) compared to Sham and CLP. Thus, we conclude from these preliminary results that the decrease in neuropsychiatric condition is not correlated with cell number of lymphocytes. Support: FAPESP 2012/07898–0