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Effect of low level laser therapy on lung mechanics and inflammatory response
Author(s) -
Cury Vivian,
Lima Thais,
Ariga Suely,
Barbeiro Denise,
Pinheiro Nathalia,
Prado Carla Maximo,
Moretti Ana Iochabel,
Souza Heraldo Possolo
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb445
Subject(s) - low level laser therapy , bronchoalveolar lavage , medicine , tumor necrosis factor alpha , inflammation , lung , laser therapy , laser , physics , optics
Low level laser therapy (LLLT) is currently prescribed as an adjuvant therapy for inflammatory diseases, however its use in internal viscera is controversial. Therefore, we assessed the effect of LLLT on acute lung injury (ALI) induced by intratracheal LPS instillation. C57 black mice (n=10) were exposed to intratracheal LPS (5mg/kg) or PBS. Six hours after instillation, both groups (PBS and LPS) were irradiated with laser at 660 nm, power output 30mW, fluency 10J/cm 2 Twenty‐four hours later, bronchoalveolar lavage was collected; lungs were submitted to functional analysis and quantitation of tumor necrosis factor (TNF‐α), interleukin (IL) 1 β and intercellular adhesion molecule 1 (ICAM ‐1). LPS instillation induced infiltration of large number of inflammatory cells to distal airways, which was reduced by LLLT (2.92±0.34 vs 1.63±051 mononuclear cells; 2.25± 0.84 vs 0.43±0.19 neutrophils; p<0.05). We also observed that LLLT was efficient in reducing secretion of TNF‐α, IL‐1β and ICAM‐1. In spite of reducing inflammation, LLLT treatment did not affect lung mechanical properties, since no change was observed in tissue resistance or elastance. Therefore, we can conclude that LLLT is able to reduce inflammatory reaction in lungs exposed to LPS, without affecting the pulmonary function and recovery.