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Intestinal ROS is reduced in mice after fasting
Author(s) -
Carlsen Harald,
Kielland Anders,
Blomhoff Rune
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb418
Subject(s) - ileum , jejunum , duodenum , small intestine , reactive oxygen species , medicine , digestion (alchemy) , chemistry , biology , endocrinology , biochemistry , chromatography
The intestine contains both red‐ox regulating systems and numerous enzymes producing reactive oxygen species (ROS). It is reasonable to assume that intestinal ROS content is related to the function of the intestine: food digestion. To shed some light on this we have evaluated the effect of fasting and diet change on intestinal ROS. Mice were fasted over night and ROS was evaluated by use of the bioluminescent probe L‐012. This probe emits photons when reacting with ROS, which can be detected by optical imaging. L‐012 was injected i.p. five minutes prior to sacrificing the animals and dissection of the intestine for imaging. Bioluminescence was recorded from duodenum, jejunum, ileum and colon. We found little effect of fasting in duodenum and colon; however, in jejunum and ileum we observed a large reduction in the L‐012 signal. Furthermore, we fed mice a typical unhealthy diet (western diet) for two months. This caused a significant reduction in the L‐012 signal. In conclusion, this suggested that the ROS content in lower part of small intestine is dependent on the presence of specific food components.