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Antioxidant and antigenotoxic properties of water extract of sulfur fed duck
Author(s) -
Park Eunju,
Choi MiJoo,
Seo BoYoung
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb393
Subject(s) - antioxidant , oxygen radical absorbance capacity , chemistry , trolox , dpph , oxidative stress , sulfur , hydrogen peroxide , trolox equivalent antioxidant capacity , food science , hydroxyl radical , ferric , dna oxidation , biochemistry , traditional medicine , organic chemistry , oxidative damage , medicine
Duck fed sulfur has been traditionally used in alternative medicine as a detoxifying agent in Korea. This study was carried out to investigate the antioxidant and antigenotoxic properties of hot water extract of sulfur fed duck (SFD). The antioxidant activities of SFD were measured by DPPH radical scavenging activity, oxygen radical absorbance capacity (ORAC) and cellular antioxidant activity (CAC) values. Non cellular antioxidant activities increased in dose dependent manners, indicating the DPPH radical scavenging activity of 86.6% at 1000 μg/mL and ORAC value of 2.8±0.6 mM trolox equivalent (TE) at 50 μg/mL of SFD. The AAPH − induced oxidative stress in HepG2 cells was significantly inhibited by 17.1, 22.1, and 29.4% at the concentrations of 1, 5, and 10 μg/mL of SFD, respectively. Human leukocyte DNA damage induced by 200 μM of hydrogen peroxide, 200 μM of 4‐hydroxynonenal (HNE), or 250 μM of ferric nitrilotriacetate (FeNTA) was effectively protected by the pre‐treatment of SFD at the concentrations of 1–50 μg/mL. Our data provide evidence that support the usage of sulfur fed duck in traditional medicine in terms of antioxidant and antigenotoxic effect.