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Hypoxia in pressure overloaded heart
Author(s) -
Nanka Ondrej,
Pesevski Zivorad,
Stopkova Tereza,
Sedmera David
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb37
Subject(s) - interventricular septum , pressure overload , medicine , anatomy , hypoxia (environmental) , thickening , ventricular pressure , cardiology , circulatory system , heart development , hemodynamics , biology , embryonic stem cell , chemistry , muscle hypertrophy , ventricle , organic chemistry , biochemistry , cardiac hypertrophy , polymer science , oxygen , gene
The heart grows in response to circulatory demands and this growth is governed by genetic programs but may be modified by hemodynamic alterations. Hypoxic regions are present in the developing myocardium. Conotruncal banding (CTB) is a well‐established model of embryonic pressure overload. The aim of this study was to elucidate how the extent of hypoxic regions in myocardium is changed under pressure overload. Conotruncal banding was performed in the chick model at embryonic day (ED) 4. The marker for hypoxia Hypoxyprobe 1, was injected into the vitelline vein at ED 6 and ED 8. The embryos were reincubated for 1h and then fixed in 4% PFA. The reaction product was detected on paraffin sections with Hypoxyprobe‐1 antibody. Examination of control hearts showed isolated regions of hypoxia in the left ventricular wall and interventricular septum. In pressure‐overloaded hearts we found ventricular dilatation, thickening of the compact myocardium and trabeculae. The hypoxic regions were significantly more extensive in the compact layer of both ventricles, septum, and the outflow tract. We conclude that the thickening of the embryonic myocardium induced by pressure overload results in its increased hypoxia. This finding can explain previous observations of matching capillary growth in this model, as well as accelerated maturation of the conduction system. Supported by project UNCE 204013 and PRVOUK P35/LF1/5.