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Application of Urine Metabolomics to Probe the Effects of Long‐Term Bed Rest as a Model for Spaceflight
Author(s) -
Ericson Karen Louise,
Cooper B. R.,
Peels M. E.,
Deng Y.,
Smith S. M.,
Coburn S. P.
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb311
Subject(s) - spaceflight , metabolomics , urine , bed rest , muscle mass , chemistry , biomarker , biomarker discovery , urinary system , chromatography , medicine , proteomics , engineering , biochemistry , aerospace engineering , gene
This project aims to determine if urine metabolomics can be a meaningful approach to assess spaceflight health effects. Muscle loss in spaceflight might be caused by decreased muscle synthesis or increased muscle breakdown, and identification of an appropriate biomarker might suggest appropriate countermeasures. In this study, urine samples from 8 subjects in a bed rest study (paired before and during bed rest) were analyzed using hydrophilic interaction liquid chromatography (HILIC) and positive ionization mode time‐of‐flight mass spectrometry. Data analysis indicated measurable differences in urinary compounds of the paired groups. Of 1873 unique compounds found, 20 were identified as having high significance (consistent increase or decrease during the sample period) and were thus considered possible biomarkers. Comparison of the bioinformatics programs used here, Agilent Mass Profiler Professional and Purdue's in‐house Omics Discovery Pipeline, could be valuable for future metabolomic studies. Identification of high‐significance compounds is continuing. Support: Grant 10465 – Indiana Space Grant Consortium & Indiana University‐Purdue University Fort Wayne Office of Research & External Support (KLE).