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Characterization and distribution of the zinc transporter, Zip14, along the murine GI tract
Author(s) -
Guthrie Gregory J,
Cousins Robert J
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb280
Subject(s) - zinc , transport protein , cytoplasm , extracellular , chemistry , transporter , microbiology and biotechnology , jejunum , biochemistry , biology , gene , organic chemistry
ZIP14 is a member of the SLC39 family of transmembrane zinc transporters, which increase zinc content in the cytoplasmic compartment of the cell either from extracellular influx or organellar efflux of zinc. Within the intestines, zinc transport also displays segment‐specific uptake with higher transport of zinc in the duodenum and jejunum. Intestinal zinc transporters, such as ZIP4, also display dynamic localization to the plasma membrane under specific cellular conditions. ZIP14 is responsive to endotoxin, yet changes in localization due to endotoxin are unknown. ZIP14 localization and its function in both cytoplasmic zinc exchange and site‐specific transport are the aims of this study. Isolated segments of the G.I. tract were used to determine protein distribution of ZIP14. ZIP14 protein is most highly distributed along the duodenum followed by jejunum. For intestinal localization, mice were injected with either PBS, or lipopolysaccharide (LPS, 2 mg/kg). Cytoplasmic and membrane fractions were isolated and protein was used to determine ZIP14 localization. The basolateral membrane shows the greatest expression of ZIP14. Intestinal uptake studies using 65 Zn (3μCi, subcutaneous) were performed with either PBS, or LPS (2mg/kg, intraperitoneal). 65 Zn uptake in the intestine is higher in the ZIP14 −/− compared to ZIP14 +/+ genotype. This research is supported by NIH Grant DK94244