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Malvidin and delphinidin exhibit a dose‐dependent effect on cell viability and apoptosis in HT‐29 cells
Author(s) -
Krauss Amy,
Fischer Joan
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb274
Subject(s) - apoptosis , viability assay , delphinidin , chemistry , cell , microbiology and biotechnology , cell growth , biology , biochemistry , antioxidant , cyanidin
Anthocyanidins (ANTH) induce apoptosis in some cancer cell lines, but studies describing the dose‐dependent effects of ANTH on cell populations are limited. The objective of this study was to evaluate the dose‐dependent response of malvidin (MAL) and delphinidin (DEL), ANTH with different chemical structures, on cell viability and apoptosis in HT‐29 cells in comparison with curcumin (CUR), a phenolic compound and a known inducer of apoptosis. Cells were treated with 0, 5, 10, 25, 50, 80, or 100 umol/L MAL or DEL or 40 or 80 umol/L CUR for 48 h at 37°C, 5% CO2, 95% air. Cells were treated with 250 umol/L tert‐butyl hydroperoxide for 2 h as a positive control. A significant ANTH concentration effect on cell proliferation and apoptosis was observed but MAL and DEL had similar effects on these measures. Lower concentrations of DEL (5 and 10 umol/L) and MAL (5 umol/L) increased cell viability by 12–16% compared to control, while higher concentrations of CUR, (80 umol/L), MAL (80 umol/L and 100 umol/L), and DEL (80 umol/L and 100 umol/L) decreased cell viability by 16–44%. Apoptosis was assessed by measuring caspase‐3 activity. Caspase‐3 activity only significantly increased with 80 umol/L MAL and DEL, by a fold increase of about 1.7. This data suggests MAL and DEL have comparable effects on HT‐29 cell viability and concentrations as high as 80 umol/L MAL and DEL are needed to induce apoptosis.