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Pleiotrophin loaded halloysite nanoparticles as chemoattractants for osteoblasts
Author(s) -
Banda Nikhileshwar,
Mills David
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb27
Subject(s) - halloysite , osteoblast , pleiotrophin , chemistry , nanoparticle , growth factor , microbiology and biotechnology , materials science , nanotechnology , in vitro , biology , composite material , biochemistry , receptor
Tissue engineering is being seen as the ultimate solution to replace tissues which are seriously damaged. Damaged bones in certain disorders may take prolonged time to heal by body's natural mechanisms. The osteoblasts regenerate the bone by producing the osteoid matrix and mineralizing it. The bone healing process can be accelerated if more osteoblasts are employed at the site of bone damage. This study's principal intention is to determine whether the halloysite clay nanotubes would attract osteoblasts when loaded with growth factors as chemotactic clues. The halloysite clay nanoparticles vacuum‐loaded with the growth factor pleiotrophin and osteoblasts are to be dispersed in the collagen hydrogel. The osteoblasts in the hydrogel construct will be periodically assessed for cell proliferation and mineralization by conducting assays during the cell culture. If the hypothesis is correct, the proximity between halloysites and osteoblasts should increase due to the latter's movement. Also the osteoblast cell proliferation should occur in the direction of the halloysite and the resulting cells should grow and differentiate close to the halloysite nanoparticles’ surface. Funding provided by the State of Louisiana Governor's Biotechnology Initiative.