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Mast cell proteases 6 and 7 show differential effects on endothelial cells in the induction of tube formation and the release of angiogenic factors
Author(s) -
Junior Devandir Antonio Souza,
Borges Antonio Carlos,
Santana Ana Carolina,
Oliver Constance,
Jamur Maria Celia
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb233
Subject(s) - proteases , angiogenesis , microbiology and biotechnology , mast cell , tryptase , matrix metalloproteinase , protease , endothelial stem cell , biology , chemistry , in vitro , immunology , biochemistry , enzyme , cancer research
An association between mast cells and angiogenesis is known to exist, but the exact role that mast cells play in this process is still unclear. It is thought that the mediators released by mast cells are important in neovascularization. However, it is not known how individual mediators are involved in this process. The present study aims to understand the way in which specific subtypes of recombinant mouse mast cell proteases (rmMCP‐6 and 7) induce tube formation by endothelial cells (SVEC4–10). SVEC4–10 cells were cultured on GeltrexTM with either rmMCP‐6 or 7 and tube formation was analyzed by phase contrast microscopy and scanning electron microscopy (SEM). Additionally, the capacity of the subtypes of proteases to induce endothelial cells to produce pro‐and anti‐angiogenic cytokines and proteins was analyzed. Our results show that rmMCP‐6 and 7 are able to induce tube formation in SVEC4–10 cells. SEM shows that following protease treatment SVEC4–10 cells penetrating the gel. Furthermore, rmMCP‐6 and rmMCP‐7 were able to induce the differential release of angiogenic factors. rmMCP‐7 was more efficient in inducing tube formation and releasing angiogenic factors than rmMCP‐6. However, the expression and activity of metalloproteases were not altered by incubation with these mast cell proteases. This study suggests that subtypes of proteases released from mast cells may be important in angiogenesis.