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Protein Kinase C Isoforms are Expressed During Intestinal Regeneration in Sea Cucumber Holothuria glaberrima .
Author(s) -
Rosado Edwin Antonio,
García José E
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb227
Subject(s) - gene isoform , biology , protein kinase c , microbiology and biotechnology , evisceration (ophthalmology) , kinase , regeneration (biology) , protein kinase domain , sea cucumber , biochemistry , gene , mutant , pathology , medicine , alternative medicine , ecology
Molecular and cellular studies on the intestinal regeneration process of the sea cucumber Holothuria glaberrima allows us to understand how cells are rearranged and assembled into a functional organ. Protein kinases c (Pkcs), a superfamily of serine/threonine kinases, have been implicated in a wide range of cellular processes and in the regulation of growth, apoptosis, and differentiation in a variety of cell types. We now report the characterization of at least four Pkc isoforms from regenerating intestine cDNA libraries of H. glaberrima . One of the Pkc isoform showed similarity to the PB1 domain required for the enzymatic activity of atypical Pkcs (identity >; 63%). Another three Pkc isoforms showed the highest similarity to the C1 activation domain of the classical Pkc isoforms (identity >; 46%). Two of the Pkcs, one classical and one atypical isoform, were significantly upregulated in the regenerating intestine from early to middle stages of regeneration (3–14 days post‐evisceration) compared to normal uneviscerated intestine where its expression was almost negligible. The increase in gene expression coincides with drastic dedifferentiation, intense cellular division, and apoptosis events that take place during early to middle stages of regeneration implicating Pkcs as being key modulators of these processes. Research supported by NIH Grant Number 5T34GM007821–33.