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Lysophosphatidic Acid Regulates uPAR Expression and Localization
Author(s) -
Ciota Alexandra M,
Kobliska Amy,
Marsh Sydney,
Ellerbroek Shawn M
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb187
Subject(s) - lysophosphatidic acid , urokinase receptor , chemistry , cell , ovarian cancer , messenger rna , cancer research , cell migration , receptor , cell culture , microbiology and biotechnology , biology , cancer , medicine , biochemistry , gene , genetics
Lysophosphatidic acid (LPA) stimulates in vitro invasion of ovarian epithelial cancer cells through a uPA‐dependent process. We therefore analyzed the effects of LPA on the cell surface uPA receptor (uPAR). While both uPAR mRNA and protein expression was consistent with control after 2 hours of LPA stimulation, LPA strongly stimulated uPAR redistribution into punctate aggregates or to cell junctions, depending on cell line. After 24 hours of stimulation, uPAR mRNA levels increased 4.0±2.3 fold in OVCA 429 cells and were modestly impacted in OVCA 433 cells (1.8±0.5 fold). In accordance, cell surface‐localized uPA activity increased 4.3±2.5 fold (OVCA 429) or 2.1±0.9 fold (OVCA 433) and was accompanied by increased uPAR immunofluorescence staining. At this same time, mRNA levels of uPA and PAI‐1 were not significantly changed. These data provide mechanistic insight into the actions of a lipid that positively correlates with ovarian cancer progression.