Premium
Investigating the targeting and regulation of Ded1, a DEAD‐box ATPase, in returning repressed mRNAs to translation
Author(s) -
Pollard Beth,
Hilliker Angela
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb151
Subject(s) - dead box , biology , translation (biology) , microbiology and biotechnology , messenger rna , helicase , rna , biochemistry , gene
Translation regulation is important, especially during stress, in neurons, and in early development, where many mRNAs are stored in translationally repressed states. These repressed mRNAs can be returned to translation, but this process is poorly understood. We have previously shown evidence that the RNA helicase, Ded1, performs two sequential functions to return a repressed mRNA back to translation in an ATP‐dependent manner. First, Ded1 promotes the formation of a “pre‐48S mRNP” that includes the initiation factors eIF4F and Pab1. Then, Ded1 hydrolyzes ATP, allowing the mRNA to become translated. We hypothesize that Ded1 acts as an ATP‐dependent switch to return mRNAs back to the translating pool. We are investigating how Ded1 forms this pre‐48S intermediate. Ded1's ability to form this repressed mRNP in vitro depends on the presence of a polyA tail. Additionally, Ded1 increases the recruitment of Pab1 to the m7G cap. We are currently investigating the relationship between Pab1 and Ded1 in formation of this repression complex.