Premium
Metalloproteomic approach to the determination of calcium, iron and zinc bound to secretory immunoglobulin A in human milk
Author(s) -
Pozzi Carla Mariane Costa,
Braga Camila Pereira,
Vieira José Cavalcante Souza,
Okoshi Marina Politi,
Magalhães Padilha Pedro
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.lb141
Subject(s) - chemistry , polymeric immunoglobulin receptor , zinc , secretory component , electrospray ionization , calcium , mass spectrometry , chromatography , metal , antibody , biochemistry , receptor , biology , immunology , organic chemistry
At this present work, the presence of calcium, iron and zinc bound to secretory immunoglobulin A (sIgA) from human milk was investigated. The sIgA components were separated by two‐dimensional electrophoresis (2D‐PAGE) and they were identified by electrospray ionization mass spectrometry (ESI/MS/MS). A train of eight spots with 5.4