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A Systematic Study of the Chemical Stress Response Induced by Bleach And Vinegar in E. Coli on Viability and Protein Expression Profiles Using Plating Assays And Matrix‐Assisted Laser Desorption Ionization (MALDI) Biotyper™ System
Author(s) -
Liu Serena,
VardarUlu Didem
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.997.1
Subject(s) - contamination , escherichia coli , chemistry , matrix (chemical analysis) , bleach , plating (geology) , desorption , food science , microbiology and biotechnology , incubation , chromatography , environmental chemistry , biology , biochemistry , adsorption , ecology , organic chemistry , paleontology , gene
Bacterial water contamination is a serious global public health problem. Even though most mammals carry Escherichia coli (E. coli) in their guts, certain strains can lead to severe ailments of the gastro‐intestinal tract including diarrhea, the second leading cause of death in children under five. While the contamination frequently originates at the water source, water storage conditions can heavily exacerbate the health risk. Here, we present our systematic study of the effects of two ordinary household chemicals, bleach and vinegar, on E. coli viability and protein expression profiles, as a function of chemical concentration, contact time, E. coli density, and incubation temperature, A combination of standard culture plating assays and spectroscopic measurements were used to assess viability and to determine the minimal chemical concentration required to keep bacterial levels below the EPA's safe drinking water limits. Matrix‐assisted Laser Desorption Ionization (MALDI) Biotyper was used to compare the unique molecular fingerprint of E. coli subjected to multiple chemical stress conditions and harvested at different time points. This work represents the initial step in identifying E. coli proteins sensitive to chemical treatment and could provide insights into the mechanism of stress response in E. coli that would be important in developing strategies to overcome bacterial resistance.