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Isoflurane preconditioning involves the upregulation of aquaporin genes
Author(s) -
Schwamb Zackary,
Seidler Norbert,
Theisen Christopher
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.996.8
Subject(s) - isoflurane , downregulation and upregulation , chemistry , anesthetic , aquaporin , ischemic preconditioning , homeostasis , pharmacology , gene expression , aquaporin 1 , microbiology and biotechnology , gene , anesthesia , biochemistry , biology , medicine , ischemia , water channel , mechanical engineering , engineering , inlet , organic chemistry
Anesthetic preconditioning is a phenomenon in which cells that have been previously exposed to volatile anesthetics exhibit protection from future cellular insult. The mechanism of isoflurane‐induced changes in gene expression is thought to involve the dehydration of proteins, which consequently leads to protein misfolding and an upregulation of protein chaperones including alphaA‐crystallin. In the present study we found that the neuronal cell line SH‐SY5Y exhibited increased expression of the genes for aquaporins 1, 4, 5, and 8, 48 hr after exposure to isoflurane. The levels of aquaporin 1 also remained elevated 72 hr after exposure which coincided with increases in alphaA‐crystallin expression. We also found that isoflurane exposure induced a preconditioning effect that was exhibited by an increase in SH‐SY5Y cell viability in the presence of ethanol, 72 hr after exposure to the anesthetic. These data indicate that isoflurane preconditioning is protective against dehydration stress in a cell culture model and may involve the regulation of water homeostasis via aquaporin channels.