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Identification of SCAL1, a novel long non‐coding RNA, induced by cigarette smoke and associated with lung cancer
Author(s) -
Thai Philip,
Statt Sarah,
Liang Ellen,
Campbell Caitlin,
Wu Reen
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.983.1
Subject(s) - lung cancer , long non coding rna , rna , biomarker , biology , cancer research , cancer , gene , exon , medicine , pathology , genetics
Lung cancer remains the largest cause of cancer related deaths. Despite numerous gene profiling studies on smoke induced injury in the airways. A novel type of regulatory RNA termed long non‐coding RNA (lncRNA) have been identified in recent years by RNA sequencing. Thus far, limited studies have looked at lncRNAs and lung cancer. Objective Determine if specific lncRNAs are associated with lung cancer and can be used as a biomarker for early diagnosis. Methods and Results A non‐metastatic prone lung cancer cell line CL1–0 was compared to its derived invasive subtype CL1–5 by RNA sequencing. A specific lncRNA was identified that was highly expressed in the CL1–5 compared to CL1–0 cells. We have termed this gene SCAL1 and found it to be elevated in numerous other lung cancer cell lines compared to normal bronchial epithelial cells. Subsequent studies revealed this lncRNA is located on chromosome 5 and has 4 putative exons with evidence of evolutionary conservation. In normal bronchial epithelial cells, cigarette smoke extract can induce this lncRNA. Studies with siRNA and chromatin immunoprecipitation (ChIP) revealed that SCAL1's expression is NRF2 dependent. Current studies are underway to determine the functional role of this lncRNA in normal and cancer cells. Conclusions SCAL1 is a lncRNA that may represent a novel biomarker of smoke induced lung cancer. Supported by NIH RO1HL096373–01A1 (RW).