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The role of neuropeptide Y and aquaporin 4 in the pathogenesis of intestinal dysfunction caused by traumatic brain injury
Author(s) -
Duan Hubin,
Hao Chunyan,
Li Shuzhen,
Fan Yimin,
Wang Hongqin,
Hao Jiehe,
Liu Yueting,
Liu Xiaodong
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.981.1
Subject(s) - pathogenesis , neuropeptide y receptor , medicine , traumatic brain injury , endocrinology , pathology , immunohistochemistry , jejunum , intestinal mucosa , pathological , gastroenterology , neuropeptide , receptor , psychiatry
Backgroud and purpose To study the role of neuropeptide Y(NPY) and aquaporin 4 (AQP4) in the pathogenesis of intestinal dysfunction caused by traumatic brain injury£TBI£©. Methods 40 Wistar rats were randomly divided into control group, mild trauma group, moderate trauma group and severe trauma group. All the rats were sacrificed at 24 hours after the operation. The contents of NPY and AQP4 in the rats blood and jejunum were detected by ELISA, PCR, western blot and immunohistochemical staining. The pathological changes of intestinal cells structure and ultrastructure were observed by light microscopy and transmission electron microscopy. Results The more severe the grade of trauma, the more serious the degree of intestinal mucosal injury, the higher the content of NPY and AQP4 in the plasma and jejunum of rats(P<0.05). Moreover, there are positive correlation between NPY and AQP4(P<0.05). Conclusion AQP4 and NPY may be involved in the pathogenesis of intestinal dysfunction after TBI. Increased NPY level might be responsible for the Intestinal ischemia and hypoxia, AQP4 might play an important role in intestinal edema. In addition, the increase of NPY content might be one of the main reasons that lead to the increased of AQP4 content after TBI.