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Effects of recognition sequence variations on transcription regulation of multidrug resistance regulator Pdr1p in yeast
Author(s) -
Nishida Nao,
Kuroda Kouichi,
Ueda Mitsuyoshi
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.980.6
Subject(s) - promoter , transcription factor , gene , transcription (linguistics) , biology , genetics , multiple drug resistance , upstream activating sequence , response element , yeast , e box , gene expression , drug resistance , linguistics , philosophy
For precise transcription regulation, it is important to recognize specific target genes from the genome. Pdr1p is a key transcription factor of multidrug resistance, also involves in organic solvent tolerance in yeast [1]. Pdr1p is one of the transcription factors which recognize pleiotropic drug resistance elements (PDREs). PDRE is 8 bases long, and has known 5 sequence variations, which functional differences are not known. ABC transporters responsible for multidrug resistance harbor PDREs in their promoters, while cell wall proteins responsible for organic solvent tolerance do not [2]. Some one point mutations in Pdr1p cause upregulation of downstream genes, resulting in multidrug resistance. However, from our previous studies, not all genes sharing PDRE at their promoters are regulated in the same way. We changed PDREs in a native promoter to each sequence variation and evaluated downstream transcription levels. We showed that PDRE variations have some effect on transcription levels of downstream genes. The results suggest that PDRE sequence variation may contribute to precise transcription regulation by Pdr1p, by affecting binding affinity or cofactor bindings [3].