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Study on the neurogenic mechanisms of traumatic brain injury
Author(s) -
Duan Hubin,
Fan Yimin,
Hao Chunyan,
Li Shuzhen,
Wang Suping,
Wang Hongqin,
Kuai Dong,
Lian Shizhong,
Xue Naizhao
Publication year - 2013
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.27.1_supplement.980.10
Subject(s) - traumatic brain injury , neurogenic inflammation , medicine , neuropeptide y receptor , inflammation , neuropeptide , substance p , immunohistochemistry , edema , western blot , endocrinology , pathology , anesthesia , biology , gene , receptor , psychiatry , biochemistry
Backgroud and purpose In recent years, the mechanism of neurogenic inflammation in traumatic brain injury (TBI) gradually attracted people's attention. The purpose of research is to explore the specific mechanisms of traumatic brain edema caused by TBI. Methods 40 male Wistar rats were randomly divided into control group, mild trauma group, moderate trauma and severe trauma group. Discharge frequency of the thalamic ventral posterior medial nucleus (VPM) were recorded after TBI.The expression of neuropeptide Y (NPY), substance P (SP), aquaporin‐4 (AQP4) and nuclear transcription factor‐kappa B (NF‐¦ÊB) were detected by immunohistochemical, Western blot, RT‐PCR and ELISA. Results With the increase of trauma degree, the more obvious cerebral edema, the more visible neurotransmitters release and inflammatory cell infiltration between the synapses, the more gene and protein expression of SP, NPY, NF‐¦ÊB, and AQP4. There are positive correlations between the above gene and protein expression; There are also positive correlations between SP and the firing frequency of VPM.(P <0.05). Conclusion The more severe damage of TBI, the more gene and protein expression of above neuropeptides. Neurogenic inflammation and neurogenic mechanisms play an important role in traumatic brain injury.